DNA fragmentation assay
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DNA fragmentation assayCSNL 2024. 2. 16. 11:54
DNA fragmentation assay 1. Centrifuge medium at 2500rpm for 10min 2. Add 750 μl of lysis buffer into 60mm cell culture plate (100mm cell culture plate의 경우에는 1 ml의lysis buffer를 첨가한다.) 3. Stand at 4℃ for 30min 4.Transfer centrifuged cells (pellets : step 1) into 60mm cell culture plate (step2) 5.Scrap with cell scraper 6.Mix cells up and down with pipette (1000P) 7.Transfer to E-tube 8.Centrifuge ..