BONOFACTORY BONOFACTORY

Homologous Recombination Method (and Knockout Mouse) When an investigator wants to replace one allele with an engineered construct but not affect any other locus in the genome, then the method of choice is homologous recombination. To perform homolgous recombination, you must know the DNA sequence of the gene you want to replace (figure 1). With this information, it is possible to replace any ge..